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qPCR is Value-productive and accessible as the laboratory settings, machinery and reagents are just like Those people desired for making ready the NGS libraries.
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The qPCR primers have marginally decrease but sufficiently similar coverage for that domain Micro organism as compared to the primers for NGS. The protection of both of those qPCR and NGS primer sets is very equivalent to the four taxa chosen for taxon-certain qPCR (S1B Desk), which delivers reliable validation in the explained process Within this review. For upcoming improvement of the herein introduced solution, a qPCR assay using exactly the same primer pair as for NGS can be optimized and validated.
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